一种检测猕猴属动物TRIMCyp基因型的PCR方法

PCR method for detecting TRIMCyp genotypes of animals of macaca

Abstract

The invention discloses a PCR method for detecting the mutation of TRIM4 loca of animals of macaca, which comprises the following steps of: (1) extracting DNA from the hair of the animals of the macaca; (2) performing PCR amplification by taking SEQ ID NO:1 and SEQ ID NO:2 as primers; and (3) determining the TRIM5 loca of the animals to be detected are not mutated if an amplification product is one 2,300 to 2,400 bp segment, determining the TRIM5 loca are subjected to heterozygous mutation if the amplification products are the 2,300 to 2,400 bp segment and a 3,000 to 3,150 bp segment respectively, and determining the TRIM5 loca are subjected to homozygous mutation if the amplification product is only the 3,000 to 3,150 bp segment. The method of the invention has the advantage of accurately detecting the TRIMCyp genotypes of the animals of the macaca, and can be used for surveying the distribution of TRIMCyp in macaques in China and screening positive individuals.
本发明公开了一种检测猕猴属动物TRIM5基因座突变的PCR方法,包括:(1)从猕猴属动物毛发中提取DNA;(2)以SEQ ID NO:1和SEQ ID NO:2为引物进行PCR扩增;(3)如果扩增产物为一条2300-2400bp片段,则待检测动物TRIM5基因座没有发生突变;如果扩增产物分别为2300-2400bp和3000-3150bp的两条片段,则TRIM5基因发生杂合突变;如果扩增产物仅为一条3000-3150bp的片段,则TRIM5基因座发生纯合突变。本发明方法能够准确的检测出猕猴属动物的TRIMCyp基因型,可以用于调查TRIMCyp在中国地区猕猴中分布,筛选阳性个体。

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    《中国免疫学杂志》 20081231 刘红亮等 《平顶猴TRIM5a基因变异影响其抗HIV-1功能》 全文 1-5 第24卷, 第10期
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Cited By (1)

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    CN-102758016-AOctober 31, 2012新疆农业大学Kit for simply, conveniently and rapidly detecting sheep multifetal gene BMPR-IB (bone morphogenetic protein receptor IB)